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Part:BBa_K4204003
Designed by: Jianfei Song Group: iGEM22_BNDS_China (2022-10-04)
Transcriptional unit of sgRNA targeting HBLA gene of B.cereus
Description
This sequence contains 3 protection bases at the 5’ end of the sequence, which is to protect the sequence from damage. And immediately following the T7 promoter, connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-HBLA region pairs with the targeted B.cereus HBLA gene sequence and enables cleavage.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 26
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 26
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 26
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |